Wolinella succinogenesgrows by oxidative phosphorylation with polysul®de as terminal electron acceptor and either H2 or formate as electron donor (polysul®de respiration). The function of the respiratory chains catalyzing these reactions was investigated. Proteoliposomes containing polysul®de reductase (Psr) and either hydrogenase or formate dehy-drogenase isolated fromthemembrane fraction ofWolinella succinogenescatalyzed polysul®de respiration, provided that methyl-menaquinone-6 isolated fromW. .