Department of Molecular and Cellular Neurobiology, Nencki Institute of Experimental Biology, Warsaw, Poland; Department of Biochemistry and Molecular Biology, University of Debrecen, Hungary; 3Department of Inorganic Chemistry, and 4Research Group for Antibiotics, Department of Chemistry, University of Debrecen, Hungary; 5 International Institute of Molecular and Cell Biology, Warsaw, Poland This study characterizes the calcium-bound CR I – II domain (residues 1–100) of rat calretinin (CR). CR, with six EF-hand motifs, is believed to function as a neuronal intracellular calcium-buffer and/or calcium-sensor. .