Manganese lipoxygenase was isolated to homogeneity from the take-all fungus, Gaeumannomyces graminis. The C-terminal amino acids and several internal peptides were sequenced, and the information was used to obtain a cDNA probe by RT/PCR. Screening of a genomic library of G. graminis yielded a full-length clone of the Mn-Lipoxygenase gene. cDNA analysis showed that the gene spanned kb and contained one intron (133 bp). Northern blot analyses indicated two transcripts ( and kb). The deduced amino-acid sequence of the Mn-Lipoxygenase precursor (618 amino acids, kDa) could be aligned with mammalian and plant lipoxygenases with 23–28% identity over.
