This study investigated the chemical, physical, and mechanical properties of the bamboo species Phyllostachys pubescens and its utilization potential to manufacture medium density fiberboard (MDF). The result showed holocellulose and alpha-cellulose content increased from the base to the top portion. There was no significant variation in Klason lignin content or ash content from the base to the top portion of the bamboo. The outer layer had the highest holocellulose, alpha cellulose, and Klason lignin contents and the lowest extractive and ash contents. The epidermis had the highest extractive and ash contents and the lowest holocellulose and alpha-cellulose content