Each test was conducted using 3 replications. It was necessary to conduct additional experimentation when analyzing for alcohol-toluene extractive content and holocellulose content. The alcohol-toluene test is the starting material for many of the other experiments. Both the lignin and holocellulose content test are performed with extractive-free bamboo that is derived from the alcohol-toluene extractive test. Additionally, holocellulose is a necessary preparatory stage in order to determine the alpha-cellulose content. Alcohol-toluene Solubility of Bamboo The extraction apparatus consisted of a soxhlet extraction tube connected on the top end of a reflux condenser and joined at.