A one-gram, oven-dried sample of extractive-free bamboo was placed in a 150 mL beaker. Fifteen mL of cold sulfuric acid (72 percent) was added slowly while stirring and mixed well. The reaction proceeded for two hours with frequent stirring in a water bath maintained at 20 o C. When the two hours had expired, the specimen was transferred by washing it with 560 mL of distilled water into a 1,000 mL flask, diluting the concentration of the sulfuric acid to three percent. An allihn condenser was attached to the flask. The apparatus was placed in.
