Hemolymph osmolality of the shrimp (Litopenaeus vannamei) exposed to salinities of ppt or 1 ppt decreasing rapidly from 800 mOsm to 540 mOsm after 6 hours. Levels also dropped dramatically from 800 mOsm to 560 mOsm in shrimp exposed to 3 ppt after 6 hours and 1 sinificant difference were found between the osmolality levesl in the shrimp at before and after exposure. Hemolymph osmolality of the whiteleg shrimps changed after exposure to low salinities, showing hyper-osmoregulatory behavior in low salinities. .