A series of alpha-substituted 2’,5’-dihydroxychalcones bearing a cyanide, bromine, or methyl group at alpha-position were synthesized and evaluated for in vitro cytotoxicity against three cancer cell lines, including B16, murine melanoma, HCT116 (colon cancer), A431 (human epidermoid carcinoma), and HUVEC (human umbilical venous endothelial cell). The synthesized chalcones showed significant cytotoxicity against most of cell lines, with IC50 values ranging from to 10 µg/mL.
