Verticillatol was isolated from Litsea verticillata as a new eudesmane sesquiterpenoid. Its plane structure was determined by means of 1 H, 13 C, COSY, HMBC, HMQC spectroscopy. The relative stereostructure was deduced from NOESY data and induced solvent shift effect. Finally, its absolute configuration was known through analysis of 1H-NMR spectra of its Mosher esters.