NFκB plays an important role in the immune system in all organisms. In this study, physicochemical properties and modelling of finfish NFκBIα protein was analysed using in silico approach. Finfish species: guppy (Poecilia reticulate), Bicolor damselfish (Stegastes partitus), Channel catfish (Ictalurus punctatus), Coelacanth (Latimeria chalumnae), and Australian ghostshark (Callorhinchus milii) were used in this study.