As Vietnam has rich resources of local chicken breeds, some of them are known having a certain level of infectious disease resistance. Knowledge of the single-nucleotide polymorphism in exon 14 of the chicken Mx gene will facilitate further researches on the resistant activity of Mx gene and its use for breeding of influenza virus resistant chicken varieties. This study aimed to investigate the exon 14 polymorphisms of the Mx gene in some representative indigenous chicken breeds collected from different locals in Vietnam.