Lecture Principles of biochemistry - Chapter 15 (part 3): Carbon fixation (dark reactions)

In this part, you will know how rubisco is activated in the light and inactivated in the dark. Know how Mg 2+, pH and light are related to it activation; know how Ribulose-1,5-bisphosphate acts as a substrate and an inhibitor of rubisco; understand the oxygenase/carboxylase activities of rubisco. Know what are the products of the two reactions;. | Chapter 15 (part 3) Carbon Fixation (dark reactions) Carbon Dioxide Fixation Reductive Pentose Phosphate Cycle 6CO2+9ATP+5H20 9ADP+8Pi+6NADP++(DHAP or G3P) Ribulose-1,5-Bisphosphate carboxylase/oxygenase (rubisco) Activation of Rubisco Mg2+ plays role in binding and activating R 1,6-BP to accept CO2 Rubisco Rxn Mechanisms carboxylase oxygenase Reductive Pentose Phosphate Cycle Reduction Stage Phosphoglycerate kinase Glyceraldehyde dehydrogenase aldolase F 1,6-bisphophatase cytosol Reductive Pentose Phosphate Cycle Transketolases and Aldolases are used to make 5 carbon sugars Formation of 5 Carbon Sugars F-6-P + 2 G3P + DHAP + 3 ATP 3 R-1,5-BP + 3 ADP Regulation of RPP Cycle Thioredoxin Oxygenase Activity of Rubisco Photorespiration C4 Photosynthesis CAM Photosynthesis stoma | Chapter 15 (part 3) Carbon Fixation (dark reactions) Carbon Dioxide Fixation Reductive Pentose Phosphate Cycle 6CO2+9ATP+5H20 9ADP+8Pi+6NADP++(DHAP or G3P) Ribulose-1,5-Bisphosphate carboxylase/oxygenase (rubisco) Activation of Rubisco Mg2+ plays role in binding and activating R 1,6-BP to accept CO2 Rubisco Rxn Mechanisms carboxylase oxygenase Reductive Pentose Phosphate Cycle Reduction Stage Phosphoglycerate kinase Glyceraldehyde dehydrogenase aldolase F 1,6-bisphophatase cytosol Reductive Pentose Phosphate Cycle Transketolases and Aldolases are used to make 5 carbon sugars Formation of 5 Carbon Sugars F-6-P + 2 G3P + DHAP + 3 ATP 3 R-1,5-BP + 3 ADP Regulation of RPP Cycle Thioredoxin Oxygenase Activity of Rubisco Photorespiration C4 Photosynthesis CAM Photosynthesis . | Chapter 15 (part 3) Carbon Fixation (dark reactions) Carbon Dioxide Fixation Reductive Pentose Phosphate Cycle 6CO2+9ATP+5H20 9ADP+8Pi+6NADP++(DHAP or G3P) Ribulose-1,5-Bisphosphate carboxylase/oxygenase (rubisco) Activation of Rubisco Mg2+ plays role in binding and activating R 1,6-BP to accept CO2 Rubisco Rxn Mechanisms carboxylase oxygenase Reductive Pentose Phosphate Cycle Reduction Stage Phosphoglycerate kinase Glyceraldehyde dehydrogenase aldolase F 1,6-bisphophatase cytosol Reductive Pentose Phosphate Cycle Transketolases and Aldolases are used to make 5 carbon sugars Formation of 5 Carbon Sugars F-6-P + 2 G3P + DHAP + 3 ATP 3 R-1,5-BP + 3 ADP Regulation of RPP Cycle Thioredoxin Oxygenase Activity of Rubisco Photorespiration C4 Photosynthesis CAM Photosynthesis stoma | Chapter 15 (part 3) Carbon Fixation (dark reactions) Carbon Dioxide Fixation Reductive Pentose Phosphate Cycle 6CO2+9ATP+5H20 9ADP+8Pi+6NADP++(DHAP or G3P) Ribulose-1,5-Bisphosphate carboxylase/oxygenase (rubisco) Activation of Rubisco Mg2+ plays role in binding and activating R 1,6-BP to accept CO2 Rubisco Rxn Mechanisms carboxylase oxygenase Reductive Pentose Phosphate Cycle Reduction Stage Phosphoglycerate kinase Glyceraldehyde dehydrogenase aldolase F 1,6-bisphophatase cytosol Reductive Pentose Phosphate Cycle Transketolases and Aldolases are used to make 5 carbon sugars Formation of 5 Carbon Sugars F-6-P + 2 G3P + DHAP + 3 ATP 3 R-1,5-BP + 3 ADP Regulation of RPP Cycle Thioredoxin Oxygenase Activity of Rubisco Photorespiration C4 Photosynthesis CAM Photosynthesis .

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