Chapter 15 - Gene regulation in prokaryotes. This chapter include the contents: Overview of prokaryotic gene regulation, the regulation of gene transcription, attenuation of gene expression: termination of transcription, global regulatory mechanisms, a comprehensive example: the regulation of virulence genes in V. cholerae. | PowerPoint to accompany Genetics: From Genes to Genomes Fourth Edition Leland H. Hartwell, Leroy Hood, Michael L. Goldberg, Ann E. Reynolds, and Lee M. Silver Prepared by Mary A. Bedell University of Georgia Copyright © The McGraw-Hill Companies, Inc. Permission required to reproduce or display Hartwell et al., 4th edition How Genes Are Regulated PART V Copyright © The McGraw-Hill Companies, Inc. Permission required to reproduce or display Hartwell et al., 4th edition, Chapter 15 Gene Regulation in Prokaryotes Overview of Prokaryotic Gene Regulation The Regulation of Gene Transcription Attenuation of Gene Expression: Termination of Transcription Global Regulatory Mechanisms A Comprehensive Example: The Regulation of Virulence Genes in V. cholerae CHAPTER OUTLINE CHAPTER RNA polymerase participates in all three phases of transcription Initiation – core RNA polymerase plus sigma (σ) factor Core has four subunits: two alpha (α), one beta (β), one beta prime (β') DNA is unwound and polymerization begins Elongation – core RNA polymerase without σ factor Continues until RNA polymerase recognizes termination signal Termination – two kinds in bacteria Rho-dependent – Rho (ρ) protein binds to RNA polymerase and removes it from RNA Rho-independent – 20 nt sequence in RNA forms stem-loop Copyright © The McGraw-Hill Companies, Inc. Permission required to reproduce or display Hartwell et al., 4th edition, Chapter 15 Role of RNA polymerase in initiation and elongation phases of transcription Copyright © The McGraw-Hill Companies, Inc. Permission required to reproduce or display Hartwell et al., 4th edition, Chapter 15 Fig. Two kinds of transcription termination in bacteria Copyright © The McGraw-Hill Companies, Inc. Permission required to reproduce or display Hartwell et al., 4th edition, Chapter 15 Fig. (cont) Regulation of expression can occur at many steps Transcriptional control Binding of RNA polymerase to . | PowerPoint to accompany Genetics: From Genes to Genomes Fourth Edition Leland H. Hartwell, Leroy Hood, Michael L. Goldberg, Ann E. Reynolds, and Lee M. Silver Prepared by Mary A. Bedell University of Georgia Copyright © The McGraw-Hill Companies, Inc. Permission required to reproduce or display Hartwell et al., 4th edition How Genes Are Regulated PART V Copyright © The McGraw-Hill Companies, Inc. Permission required to reproduce or display Hartwell et al., 4th edition, Chapter 15 Gene Regulation in Prokaryotes Overview of Prokaryotic Gene Regulation The Regulation of Gene Transcription Attenuation of Gene Expression: Termination of Transcription Global Regulatory Mechanisms A Comprehensive Example: The Regulation of Virulence Genes in V. cholerae CHAPTER OUTLINE CHAPTER RNA polymerase participates in all three phases of transcription Initiation – core RNA polymerase plus sigma (σ) factor Core has four subunits: two alpha (α), one beta (β), one
