We will begin this chapter with an examination of functional genomics. Then we will consider a quest that is even more complex than genomics: proteomics, the study of an organism’s proteome—the properties and activities of all the proteins an organism makes in its lifetime. Finally, we will introduce bioinformatics, the discipline concerned with managing and using the vast stores of data that come from genomic, proteomic, and other massive biological studies. | Molecular Biology Fifth Edition Chapter 25 Genomics II: Functional Genomics, Proteomics, and Bioinformatics Lecture PowerPoint to accompany Robert F. Weaver Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Functional Genomics: Gene Expression on a Genomic Scale Functional genomics refers to those areas that deal with the function or expression of genomes All transcripts an organism makes at any given time is an organism’s transcriptome Use of genomic information to block expression systematically is called genomic functional profiling Study of structures and functions of the protein products of genomes is proteomics 24- Transcriptomics This area is the study of all transcripts an organism makes at any given time Create DNA microarrays and microchips that hold 1000s of cDNAs or oligos Hybridize labeled RNAs from cells to these arrays or chips Intensity of hybridization at each spot reveals the extent of expression of the . | Molecular Biology Fifth Edition Chapter 25 Genomics II: Functional Genomics, Proteomics, and Bioinformatics Lecture PowerPoint to accompany Robert F. Weaver Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Functional Genomics: Gene Expression on a Genomic Scale Functional genomics refers to those areas that deal with the function or expression of genomes All transcripts an organism makes at any given time is an organism’s transcriptome Use of genomic information to block expression systematically is called genomic functional profiling Study of structures and functions of the protein products of genomes is proteomics 24- Transcriptomics This area is the study of all transcripts an organism makes at any given time Create DNA microarrays and microchips that hold 1000s of cDNAs or oligos Hybridize labeled RNAs from cells to these arrays or chips Intensity of hybridization at each spot reveals the extent of expression of the corresponding gene Microarray permits canvassing expression patterns of many genes at once Clustering of expression of genes in time and space suggest products of these genes collaborate in some process 24- Oligonucleotides on a Glass Substrate 24- Serial Analysis of Gene Expression Serial Analysis of Gene Expression (SAGE) allows us to determine: Which genes are expressed in a given tissue The extent of that expression Short tags, characteristic of particular genes, are generated from cDNAs and ligated together between linkers These ligated tags are then sequenced to determine which genes are expressed and how abundantly 24- SAGE 24- Cap Analysis of Gene Expression (CAGE) CAGE gives the same information as SAGE about which genes are expressed and how abundantly, in a given tissue It focuses on the 5’-ends of mRNAs, which allows for the identification of transcription start sites and may help in locating pormoters 24- Whole Chromosome Transcription Mapping High density whole .
