The crisis of energy producing molecules (fuels) is expected to increase in future, which is currently produced from crude mineral oil. Biodiesel is most reliable, non-toxic, biocompatible liquid fuel that can replace the existing unsustainable sources of energy. | OnLine Journal of Biological Sciences Original Research Paper Effect of Salinity, pH, Light Intensity on Growth and Lipid Production of Microalgae for Bioenergy Application Monika Prakash Rai, Trishnamoni Gautom and Nikunj Sharma Amity Institute of Biotechnology, Amity University Uttar Pradesh, Sector 125, Noida (Gautam Budh Nagar), India Article history Received: 10-06-2015 Revised: 27-07-2015 Accepted: 06-10-2015 Corresponding Author: Monika Prakash Rai Amity Institute of Biotechnology, Amity University Uttar Pradesh, Sector 125, Noida (Gautam Budh Nagar), India Tel.: +91120 4392293, Mob.: 09313887818 Email: mprai@ Abstract: The crisis of energy producing molecules (fuels) is expected to increase in future, which is currently produced from crude mineral oil. Biodiesel is most reliable, non-toxic, biocompatible liquid fuel that can replace the existing unsustainable sources of energy. Among all the known sources, microalgae display high potential for the production of biodiesel owing to their numerous benefits like higher biomass productivities than plants, no agricultural land requirement, cultivation in waste water and accumulation of 20-50% triacylglycerols. Microalgae biomass and lipid content plays an important role in commercial production of biodiesel. The present work was carried out to develop an axenic culture of a potential microalga Chlorella sp. for high biomass and enhanced lipid accumulation. The important growth parameters like pH, light colour, light intensity and photoperiod were studied for better production of Chlorella biomass. The effect of salinity on cell growth was also studied and compared with normal Fogg’s medium grown cells. The main biomolecules like carbohydrate, protein, lipid and chlorophyll content were also estimated with the help of standard biochemical methods in salt supplemented and without salt Fogg’s medium. The cellular lipid content was increased by growing the cells under different salt concentrations. The .
