Lecture Microbiology - Chapter 6: Growth and culturing of bacteria. This chapter presents the following content: Growth and cell division, bacterial growth curve, counting large populations, direct counts, counting small populations, other measurements,. | GROWTH AND CULTURING OF BACTERIA CHAPTER 6 Copyright © 2012 John Wiley & Sons, Inc. All rights reserved. Growth and Cell Division Growth Definition Binary Fission Budding Copyright © 2012 John Wiley & Sons, Inc. All rights reserved. Bacterial Growth Curve Lag phase – Adjustment to nutrients – Enzyme synthesis – DNA synthesis Log phase – Exponential cell division Stationary phase – Nutrient depletion – Waste build-up Death phase – More die than are produced – Always a few survivors Copyright © 2012 John Wiley & Sons, Inc. All rights reserved. Counting Large Populations Serial Dilutions/ Standard plate counts – Very large numbers may be counted by this method Pour plate or spread plate techniques are used Copyright © 2012 John Wiley & Sons, Inc. All rights reserved. Direct Counts Colony Counts – 300 upper limit Hemocytometer Copyright © 2012 John Wiley & Sons, Inc. All rights .
