Lecture Microbiology - Chapter 12: Sterilization and disinfection. This chapter presents the following content: Aseptic principles, controlling microbial growth, chemical antimicrobials, evaluating effectiveness, disinfectant selection criteria, mechanisms of action, classes of disinfectant,. | STERILIZATION AND DISINFECTION CHAPTER 12 Copyright © 2012 John Wiley & Sons, Inc. All rights reserved. Aseptic Principles Sterilization Disinfection – Disinfectants – Antiseptics Copyright © 2012 John Wiley & Sons, Inc. All rights reserved. Copyright © 2012 John Wiley & Sons, Inc. All rights reserved. Controlling Microbial Growth Proportional Death rates Time required for sterilization – Initial microbial numbers Role of organic matter – Cleanliness Microbial susceptibilities – Physiology – Cell wall molecules – Resistances due to enzymes Copyright © 2012 John Wiley & Sons, Inc. All rights reserved. Chemical Antimicrobials Effects on potency – – – – Time Temp pH Concentration Bacteriocidal Bacteriostatic Copyright © 2012 John Wiley & Sons, Inc. All rights .
