RULE OF THUMB: Always select the SMALLEST size pipet that will handle the volume you wish to move to achieve the greatest accuracy. Accuracy decreases as you use unnecessarily large pipets for small volumes. | 2 7 8 Micropipette Tutorial From the Office of Science Lab Technical Assistant CCC 2 7 8 This is a Micropipette it is used to accurately transfer small quantities of liquid. On this model the desired volume can be adjusted within the range of microliters (μL). Some Micropipettes have preset volumes and cannot be adjusted. This pipette will only deliver 200μL. How does it work? It works much like a syringe that would deliver an injection. Inside there is a spring loaded piston that moves up and down. 2 7 8 The plunger has two stops: - at the First Stop the piston moves through the volume that is set on the pipette. (In this case 278μL) 2 7 8 2 7 8 278μL - The second stop is extra travel used for certain circumstances like evacuating the tip, or drawing up more than the volume indicated on the pipette 2 7 8 2 7 8 278μL - Extra volume (? μL) - Find all three positions. 2 7 8 2 7 8 2 7 8 Proper Use RULE OF THUMB: Always select the SMALLEST size pipet that will handle the volume you wish to move to achieve the greatest accuracy. Accuracy decreases as you use unnecessarily large pipets for small volumes. P20 pipet: Maximum volume 20ul. Accurate between 2ul and 20ul P100 pipet: Maximum volume 100ul. Accurate between 10ul and 100ul. Deliver 15ul which pipet? 2 7 8 Set the correct volume on your micropipette by turning the adjustment knob (typically the plunger button) clockwise to increase volume or counterclockwise to decrease volume This pipette’s range is 200-1000 μL and is set to deliver 278μL Setting Volume 2 7 8 NEVER exceed the upper or lower limits of the pipette. 2 7 8 Settings can vary between models and manufacturer s. This pipette’s range is 20-100 μL and is set to deliver However you might see the following readings for this exact setting on different pipettes: Setting Volume 2 7 8 2 7 . 8 2 7 8 The first two make the decimal places obvious, while the last one implies the tenths place if you know the range. 2 7 8 Settings can vary between models . | 2 7 8 Micropipette Tutorial From the Office of Science Lab Technical Assistant CCC 2 7 8 This is a Micropipette it is used to accurately transfer small quantities of liquid. On this model the desired volume can be adjusted within the range of microliters (μL). Some Micropipettes have preset volumes and cannot be adjusted. This pipette will only deliver 200μL. How does it work? It works much like a syringe that would deliver an injection. Inside there is a spring loaded piston that moves up and down. 2 7 8 The plunger has two stops: - at the First Stop the piston moves through the volume that is set on the pipette. (In this case 278μL) 2 7 8 2 7 8 278μL - The second stop is extra travel used for certain circumstances like evacuating the tip, or drawing up more than the volume indicated on the pipette 2 7 8 2 7 8 278μL - Extra volume (? μL) - Find all three positions. 2 7 8 2 7 8 2 7 8 Proper Use RULE OF THUMB: Always select the SMALLEST size pipet that will handle the volume you wish
