Seed of rambutan as a waste of products from the canned fruit industry and was extracted by supercritical carbon dioxide (ScCO2) using CO2 as a solvent, maceration and soxhlet extraction using ethanol as the solvent,. Invite you to consult the documentation | International Journal of Chemical Engineering and Applications, Vol. 4, No. 4, August 2013 Optimization of ScCO2 Extraction of Rambutan Seed Oil Using Response Surface Methodology Nuttawan Yoswathana long time, a large amount of solvent or high temperature [7]. Therefore, novel extraction techniques with high efficiency and eco-environmental friendly are highly desirable. Supercritical carbon dioxide (ScCO2) extraction is one of novel technique which has received a great deal of attention. According to its operation, it is usually performed at low temperatures, costing short extraction time and a small amount of solvent [8], [9]. Supercritical fluids are unique in that the density is similar to a liquid while the diffusion and viscosity is between a gas and liquid [10]. Previously, ScCO2 has been used to extract flavonoids and phenolics from a wide range of plants [11]-[14], carotenoids [15], seed oil [16] and essentential oils [17]-[19]. Generally, addition of a small amount of a liquid polar modifier (methanol or ethanol) can significantly enhance extraction efficiency of flavonoids and phenolics [14]. Furthermore, ScCO2 could be easily separated from extracted substances [20]-[22]. The response surface methodology (RSM) is defined as the experimental design using the statistic method that uses quantitative data from suitable experimental design to reduce number of experimental trials needed to evaluate multiples parameters and their interactions. RSM has been achievably applied for optimizing conditions in food research [23]-[26]. This work aimed to optimize the ScCO2 extraction of oil from rambutan seed conditions, including temperature, pressure and the amount of ethanol as co-solvent using RSM. The response variable examined the yields of oil. The efficiency yield of the oil was also studied under different extraction methods. Abstract—Seed of rambutan as a waste of products from the canned fruit industry and was extracted by supercritical carbon dioxide