Objectives: To investigate the acute and subchronic toxicity of testin CT3. Method: Acute administration of testin CT3 was done as single dose from 90 g to 330 g of testin CT3 per kg/bodyweight in mice and subchronic toxicity study for 42 days was done by daily oral administration of testin CT3 at doses of and g/kg bodyweight in rabbits. | Journal of military pharmaco-medicine no7-2017 EVALUATION OF ACUTE AND SUBCHRONIC TOXICITY OF TESTIN CT3 IN EXPERIMENTAL ANIMAL Nguyen Thi Phuong Thao*; Nguyen Hoang Ngan** Vu Manh Hung**; Vu Van Dien***; Tran Cong Truong**** SUMMARY Objectives: To investigate the acute and subchronic toxicity of testin CT3. Method: Acute administration of testin CT3 was done as single dose from 90 g to 330 g of testin CT3 per kg/bodyweight in mice and subchronic toxicity study for 42 days was done by daily oral administration of testin CT3 at doses of and g/kg bodyweight in rabbits. Results: The acute toxicity study showed the LD50 of testin CT3 was g/kg ( - g/kg). The subchronic toxicity study suggested that testin CT3 with doses of g/kg/24 days and g/kg/24 days during 42 days, did not affect the increasing rabbit’s body weight; did not change the haematological parameters, ALT, AST, urea and creatinine levels; did not cause damage to liver, kidney and spleen histopathology. Conclusion: Our results suggest that the testin CT3 is relatively safe when administered orally in mice and rabbits. * Keywords: LD50; Acute toxicity; Subchronic toxicity; Testin CT3; Experimental animals. INTRODUCTION Modern medicine has gained great achievements in the treatment of spermproducing progress disorders, but most drugs have side effects due to their longlasting, relatively high cost treatment. Therefore, in recent years, many scientists tend to study traditional medicine, which shows good results, high safety, reasonable price, possible prolonged use and less adverse effects. Testin CT3 is a medicine developed from eight medicinal herbs, including Herba et Radix Eurycomae longifoliae, Fructus Cnidi, Herba Epimedii, Radix Angelicae sinensis, Fructus Tribuli terrestris, Radix Astragali membranacei, Fructus Lycii, Radix Morindae officinalis. These traditional herbal medicines are used as folk medicine for male sexual dysfunction, sperm reduction [3, 4, .
