Thí nghiệm tiến hành đánh giá khả năng tổng hợp ARNtt trong điều kiện ngập của giống lúa M202 có chứa gen chịu ngập (Sub1A). Mạ 19 ngày tuổi của hai giống lúa M202 (Sub1A) và M202 được xử lý ngập nhân tạo. Tại thời điểm trước xử lý ngập và sau xử lý 1, 3 và 7 ngày, tiến hành lấy mẫu lá và thân của hai giống lúa để đánh giá khả năng tổng hợp ARNtt bằng chỉ thị Sub1 và Adh1,. . | J. Sci. & Devel. 2015, Vol. 13, No. 8: 1382-1387 Tạp chí Khoa học và Phát triển 2015, tập 13, số 8: 1382-1387 ESTIMATION OF mRNA ACCUMULATION AND PHYSIOLOGICAL RESPONSE TRAITS ASSOCIATED WITH SUBMERGENCE TOLERANT GENE SUB1A IN RICE PLANT (Oryza sativa L.) Pham Van Cuong1*, Fukao Takeshi2, Julia Bailey-Serres2 1 Faculty of Agronomy, Vietnam National University of Agriculture Dept. of Botany and Plant Science, University of California at Riverside, The USA 2 Email*: pvcuong@ Received date: Accepted date: ABSTRACT The experiment was conducted to estimate mRNA accumulation in a submergence tolerant genotype M202 (Sub1). Nineteen-day-old seedlings of two genotypes (M202 (Sub1) and M202) were exposed to submergence. Leaves and meristems were sampled before applying submergence treatment and at 1, 3 and 7 days of submergence for estimating Sub1A and Adh1 mRNA accumulation. The results showed that the levels of mRNA increased in abundance as plants have undergone stress, especially at 3 days of submergence. The mRNA concentration also accumulated more in the meristem than in leaf tissues. The other experiment was conducted to compare the rate of recovery of photosynthesis and plant growth of the submergence-tolerant M202 (Sub1A) and the intolerant M202. Twenty three-day-old seedlings in soil-containing pots were completely submerged for up 3, 6 and 10 days (d). Photosynthetic and growth traits were measured before submergence treatment (0 day) and after 1 hour and 24 hours of recovering at 3, 6 and 10 days of submergence. When plants were prolonged under stress condition, all the photosysnthetic parameters such as photosynthetic rate, stomatal conductance, transpiration and water use efficiency decreased much more in M202 (Sub1) than those in M202. On the contrary, these parameters were able to recover in M202 (Sub1) better than in M202. Similar patterns were revealed for plant growth characters including plant .
