Bài viết Đặc tính sinh học của chủng vius PRRS KTY - 06 phân lập tại Việt Nam và đánh giá đáp ứng miễn dịch của lợn khi tiêm hỗn hợp dịch kháng nguyên virus vô hoạt trình bày: Kết quả phân tích nguồn gốc phát sinh loài dựa trên trình tự gên ORRS của chủng KTY-06 đã chỉ ra chủng virus này thuộc genotype Bắc Mỹ,. . | Vietnam J. Agri. Sci. 2016, Vol. 14, No. 10:1631-1638 Tgp ch( KH Nong nghi$p Vi^t Nam 2016, tgp 14, s6 10:1631-1638 CHARACTERIZATION OF KTY-06 FIELD PRRS STRAIN ISOLATED IN VIETNAM AND EVALUATION OF ANTIBODY PRODUCING OF THE INACTIVATED VIRUS IN PIG Nguyen Thi Lan^*, Trinh Dinh Thau\ Yamaguchi RyojiS Nguyen Van Giap\ Luong Quoc Hung\ Nguyen Thi Yen', Nguyen Huu Nani\ Nguyen Thi Hoa', Le Huynh Thanh Phuong' ' Faculty of Veterinary Medicine, Vietnam National University of Agriculture ^Faculty of Agriculture, University ofMiyazaM, Japan Email*: nguyenlan@ Received dated: Accepted date: ABSTRACT KTY-06 virus strain was isolated from 2-week-old piglet in a PRRS outbreak in Northern Vietnam in 2015 that had clinical signs, gross findings, and microsopic lesions specific with HP-PRRS. Results of a phylogenetic analysis of the open reading frame 5 region of the KTY-06 strain showed it to be a North American genotype and classified into sublineage , in which highly pathogenic Chinese PRRSV strains are also clustered. Passaging in Marc-145 cells, KTY-06 gradually induced 35% up to 100% CPE between 36 to 60 hours post inoculation. The titer of KTY-06 was X 10^ TClDso/25pl. Of which, titer of the free viaises in the supernatant was higher than the titer of the cell associated virusese. Formalin-inactivated KTY-06 was able to stimulate pigs to produce specific antibodies against PRRSV. The immune response was detectable 14 day post immunization (dpi) (S/P ratio was ± ), peaked at 42 dpi (S/P ratio was ±, ), and then declined at 49 dpi. The results suggest that KTY-06 was a candidate for strain selection for producing PRRS vaccine. Keyvirards; Biology, antigenicity, PRRSV, KTY-06, Vietnam. Dac tinh sinh hoc cua chOng virus PRRS KTY-06 phan lap t^i Viet Nam va danh gia dap ifng mien dich cua Icrn l(hi tiem hon dich Ichang nguyen virus vo hoat T6M TAT ChOng virus KTY-06 duoc ph^n lap lit Ion con .
