Bài viết Phát triển dòng lúa (Oryza sativa L.) chống chịu ngập thông qua khai thác nguồn gen Sub1 để chuyển vào giống lúa địa phương và dòng cải tiến trình bày phát triển dòng lúa chống chịu ngập hoàn toàn ở Đồng bằng sông Cửu Long đã được thực hiện suốt 3 năm thông qua dự án CLUES. 85 giống lúa cao sản và 84 dòng con lai từ tổ hợp lai hồi giao được nghiên cứu về năng suất và tính chống chịu ngập,. . | Vietnam J. Agri. Sci. 2016, Vol. 14, No. 3: 307-320 Tạp chí KH Nông nghiệp Việt Nam 2016, tập 14, số 3: 307-320 DEVELOPMENT OF RICE LINES (Oryza sativa L.) TOLERANT TO SUBMERGENCE VIA Sub1 GENE INTRODUCTION INTO LANDRACES AND ELITE BREEDING LINES Nguyen Thi Lang1*, Nguyen Thanh Hoa1, Pham Thi Thu Ha1, Nguyen Van Hieu1, Nguyen Ngoc Huong1, Bui Chi Buu2, Russell Reinke3,4, Tran Bao Toan5, Abdelbagi M. Ismail4, Reiner Wassmann4 1 Cuu Long Delta Rice Research Institute (CLRRI), Thoi Lai, Can Tho, Viet Nam 2 Institute of Agricultural Science for Southern Viet Nam (IAS), Viet Nam 3 Temperate Rice Breeder – IRRI & NICS project, Suwon 441-857, Republic of Korea 4 International Rice Research Institute, DAPO 7777, Metro Manila, Philippines 5 Biotechnology PCR Company, Can Tho, Viet Nam Email*: ntlang@ Received date: Accepted date: ABSTRACT Development of rice genotypes tolerant to complete submergence in the Mekong Delta was carried out for three continuous years by CLUES Project fund. Eighty five high-yielding cultivars and eighty four progenies from backcrossing population (BC3F3) of OM1490/IR64 Sub1 were used to study the yield components and submergence tolerance. Phenotyping was implemented at three stages: seedling, tillering and heading. Different segregation ratio and phenotypical variation indicated the complex genetic background. All genotypes have been evaluated under both submergence and non-submergence treatments. Correlation coefficient between the survival rate (%) and tiller number /10 hills was positively significant of **. Marker-assisted backcrossing (MAB) was exploited on chromosomes 9 to address ten molecular markers (SSRs). Of them, only three polymorphic SSRs really linked to the target QTLs. Markerassisted selection helped identify elite lines, which would be breeding materials via three molecular markers on the target region viz. RM3269, RM5304 and RM1367 on chromosome 9. Three selected .
