This paper focuses on optimizing the reaction condition for molar ratio of ethanol to oil, duration of reaction with 1 process and operation temperature in one step procedure to produce biodiesel fuel (BDF) from Jatropha oil. | Science & Technology Development, Vol 17, No M1 - 2014 Studies on biodiesel produced from Jatropha oil in Cambodia by a noncatalytic using C2H5OH • Nguyen Van Phuoc Institute for Environment and Resources • Chhoun Vi Thun • Pham Thanh Quan Hochiminh City University of Technology (Manuscript Received on . 2014, Manuscript Revised , 2014) ABSTRACT: Different technologies are currently available for biodiesel production from various kinds of lipid containing feedstock. Among them, the alkaline-catalyzed methods are the most widely studied. However, here are several disadvantages related to biodiesel production using alkaline catalysts such as generation of wastewater, catalyst deactivation, difficulty in the separation of biodiesel from catalyst and glycerin, etc. To limit the problems mentioned above, in this study, biodiesel is produced by a non- catalytic using C2H5OH. The effect of experimental variables (the molar ratio ethanol/oil of – , reaction times of 50 - 90 minutes and reaction 0 0 temperatures of 275 C - 295 C) on the yield of biodiesel was studied. The 96% yield of Cambodia biodiesel of reaction between C2H5OH and Jatropha Oil at 46:1 at 0 temperature 290 C at 60 minutes no using catalysts. Obtained biodiesel fuel was up to the International Standard ASTM D6751 for biodiesel fuel blend stock (B100). Key words: Biodiesel, Jatropha oil, alcoholysis, ethanol. INTRODUCTION Jatropha curcas L. is a small shrub with gray bark, white sap flows when cut. Normally plants grow 4m high and can reach a height of up to 5 meters in favorable conditions [2]. In Cambodia, they grow mainly and abundantly in a mountainous region of the north and central part, the littoral region from Banteaymeanchey province to Kampongthom province as traditional medicine and hedge such as: Battambong, Pursat, Kampongspeu, Sihanouk city, Kandal, Preyveng, Kampot, Kampongcham. Trang 102 The oil content in seeds is 30 – 50% depending on varieties, soil, .