This study introduces using simple optical system for fluorescence stimulation by UVA light to obtain in vivo facial skin fluorescence images. The red fluorescence was observed on normal skin and skin with comedones, while inflamed facial skin emitted the blue light. | SCIENCE & TECHNOLOGY DEVELOPMENT, Vol 18, 2015 Application of fluorescence technique in studying facial skin Tran Van Tien Pham Thi Hai Mien Huynh Quang Linh Ho Chi Minh city University of Technology, VNU-HCM (Manuscript Received on 30th Oct., 2015, Manuscript Revised 10th Nov., 2015) ABSTRACT This study introduces using simple emission peak at the wavelength about 630 optical system for fluorescence stimulation nm, the measured spectra showed that by UVA light to obtain in vivo facial skin protoporphyrin IX responds to red color in fluorescence images. The red fluorescence facial skin. Analysis of the fluorescence was observed on normal skin and skin with imaging and spectra can give information comedones, while inflamed facial skin about the disease state of skin and can be emitted the blue light. The fluorescence used for designing non-invasive optical devices for skin diagnosis. spectra were measured for determining the origination of facial red fluorescence. With an Key words: facial skin, fluorescence, porphyrin, protoporphyrin IX. 1. INTRODUCTION In recent years the number of people suffering from skin diseases generally and acne particularly has extremely increased due to the environmental pollution, the widespread utilization of chemicals in agriculture and food industry, etc., especially in Vietnam with the tropical climate and the major population working in the agricultural sector. Some traditional devices have usually been used in the diagnosis of skin diseases, such as facial lamp or microscope for skin surface examinations but they are not always effective, especially in earlystage diagnosis of skin disease. Recently, some new tools have been developed such as fiberbased fluorimeters – SkinScan system (Jobin Yvon, France), where fluorescence of Page 44 endogenous aminoacids is used for cutaneous lesions investigations, or DYADERM system (Biocam GmbH, Germany), which is applied for photodynamic diagnosis with .
