In this study, the content of total lipid and protein of Scylla sp. are found to be and , respectively. Additionally, trace elements contents (calcium and zink) were obtained of high level with and mg/kg, respectively. This is a potential source of materials for application in medicine and pharmaceutical industry in Vietnam. A enzymatic hydrolysis procedure was established as well in order to keep the high nutritional value of soft-shell crab materials. Based on these results, this procedure will be optimized as prospective in next step to obtain the maximized hydrolysate yield. | Vietnam Journal of Science and Technology 56 (2A) (2018) 201-208 ESTABLISHING THE HYDROLYSIS PROCEDURE OF SOFT-SHELL CRAB (Scylla sp.) IN VIET NAM Nguyen Thi Phuong Lan1, Do Thi Thanh Trung2, Van Thu Vu2, Dao Thi Dung2, Le Tat Thanh2, * 1 Graduate University of Sciences and Technology, 18 Hoang Quoc Viet, Cau Giay, Ha Noi 2 Institute of Natural Products Chemistry,VAST, 18 Hoang Quoc Viet street, Cau Giay, Ha Noi * Email: Received: 12 March 2018; Accepted for publication: 12 May 2018 ABSTRACT The mud crab (Scylla sp.) belonging to Portunidae family has been aquacultured and used to provide a lot of nutrient foods and materials for supplementary food. Recent researches showed nutrient content of soft-shell crabs is higher than that of harden crabs. In this study, the content of total lipid and protein of Scylla sp. are found to be and , respectively. Additionally, trace elements contents (calcium and zink) were obtained of high level with and mg/kg, respectively. This is a potential source of materials for application in medicine and pharmaceutical industry in Vietnam. A enzymatic hydrolysis procedure was established as well in order to keep the high nutritional value of soft-shell crab materials. Based on these results, this procedure will be optimized as prospective in next step to obtain the maximized hydrolysate yield. Keywords: Mud crab, soft-shell crab, enzymatic hydrolysis. 1. INTRODUCTION The mud crab Scylla belongs to Portunidae, Decapoda, Crustacea class, Arthropoda, and includes 4 species (S. paramamosain; S. serrata; S. olivacea and S. tranquebarica) [1, 2]. The mud crab has expanded to aquaculture in recent years due to increasing demands and rising market value of this commodity [3, 4]. Soft-shell crab is a culinary term for crabs that have recently molted their old exoskeleton and are still soft [6]. Soft-shells are removed from the water as soon as they molt to prevent any hardening of their shell.