This study was contrived for evaluating the in vitro and in vivo anti-inflammatory effects of an aqueous ethanolic leaf extract of the Vietnamese Artocarpustonkinensis A. Chev. exGagnep. usinglipopolysaccharide-stimulated RAW macrophages and a collagen antibody-induced arthritic mouse model as well. | Vietnam Journal of Science and Technology 56 (3) (2018) 286-294 DOI: INVESTIGATING THE ANTI-INFLAMMATORY ACTIVITY OF AN ETHANOLIC EXTRACT FROM ARTOCARPUS TONKINENSIS LEAVES USING A COLLAGEN ANTIBODY-INDUCED ARTHRITIC MOUSE MODEL Tran Duc Quan1, 2, Dao Duc Thien1, 2, Nguyen Thanh Tam1, 2, Nguyen Thi Hoang Anh1, 2, Nguyen Thi Cuc3, Nguyen Thi Nga3, Tran Van Sung1, Le Thi Hong Nhung4, Nguyen Hoang Sa2,5, Sabrina Adorisio6, Domenico V. Delfino6, Do Thi Thao2, 3, Trinh Thi Thuy1, 2, * 1 Institute of Chemistry,VAST, 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Viet Nam 2 Graduate University of Science and Technology, VAST, 18 Hoang Quoc Viet, Ha Noi, Viet Nam 3 Institute of Biotechnology, VAST, 18 Hoang Quoc Viet, CauGiay, Ha Noi, Viet Nam 4 HanoiUniversity of Industry, 298 Cau Dien, Bac Tu Liem, Ha Noi, Viet Nam 5 Khanh Hoa University, 01 Nguyen Chanh, Nha Trang, Khanh Hoa, Viet Nam 6 University of Perugia, Piazzale Severi, S. Andrea delle Fratte, 06132 Perugia, Italy * Email: thuy@ Received: 25 January2018; Accepted for publication: 20 April 2018 ABSTRACT This study was contrived for evaluating the in vitro and in vivo anti-inflammatory effects of an aqueous ethanolic leaf extract of the Vietnamese Artocarpustonkinensis A. Chev. exGagnep. usinglipopolysaccharide-stimulated RAW macrophages and a collagen antibody-induced arthritic mouse model as well. The obtained results here demonstrate that the 70 % ethanolic leaf extract of A. tonkinensis(AT2), traditionally used in Vietnamese folk medicine for treating arthritic symptoms, has beneficial effects on pro-inflammatory cytokine inhibition and in an experimental arthritic mouse RAW macrophages treated with AT2 showed a significant decrease in the production of IL-6 and TNF at concentrations of , 25 and 50 µg/mL (P < ), indicating its potential anti-inflammatory properties. The treatment of CAIA mice with AT2 also led to diminish the .
