DNA was isolated, then purified from pure mycelium and used to amplifying the nrLSU (nuclear ribosomal large subunit) sequence. The amplified products were used for sequencing, proof-reading by some professional solfwares before combining with other nrLSU sequences. Then this database was used to search for the suitable evolution model as well as to construct the phylogenetic trees. The results of phylogenetic analysis completely supported the morphological classification: DL0038A and DL0038B were Cordyceps takaomontana. | Journal of Science and Technology 55 (1A) (2017) 19-26 DOI: DISCOVERY OF ENTOMOPATHOGENIC FUNGI Cordyceps takaomontana AT LANGBIAN MOUNTAIN, LAM DONG, VIET NAM Dinh Minh Hiep1, Lao Duc Thuan2, Vu Tien Luyen3, Trinh Van Hanh2, Le Huyen Ai Thuy2, Truong Binh Nguyen4, * 1 Management Board of Agricultural Hi-Tech Park HCMC, Tan Phu Ward, District 9, Hochiminh City, Vietnam 2 Ho Chi Minh City Open University, 96 Vo Van Tan Street, Ward 6, District 3, Ho Chi Minh city, Vietnam 3 University of Science, VNU-HCM, 227 Nguyen Van Cu Street, Ho Chi Minh, Vietnam 4 Da Lat University, 1 Phu Dong Thien Vuong Street, Ward 8, Da Lat, Vietnam * Email: nguyentb@ Received: 30 October 2016; Accepted for publication: 30 May 2017 ABSTRACT The stromata of Cordyceps sp. were found on the Langbian Mountain – Da Lat, Vietnam at the height of meter above sea level, on the larva of Lepidoptera. Stromata were lemonyellow, clavate to elongated clavate, arising from a white pseudosclerotium. The fertile head was on the top part of stromata, darker coloured in comparison to the stipe. Perithecium was narrowly ovoid, superficial and forming dark yellow punctate on the surface of stromata. Ascus cylindrical with semi- spherical cap. Ascospores were cylindric, truncated and separately after discharge from the ascus. Pure culture was isolated on Potato Glucose Agar (PGA) medium: white colony in young and yellow in old. The isolated mycelium was not homogenous in thickness and in growth rate at the peripheral area. Conidiophores were phialide, tapering to both apexes. Conidia had elliptical shape and form into chains after maturation. DNA was isolated, then purified from pure mycelium and used to amplifying the nrLSU (nuclear ribosomal large subunit) sequence. The amplified products were used for sequencing, proof-reading by some professional solfwares before combining with other nrLSU sequences. Then this database was used to search for the suitable .
