Their structures were elucidated to be daucosterol, 1-octacosanol, docosenoic acid, methyl gallate, betulinic acid and lup-20(29)-en-3-one by 1D- and 2D-NMR spectroscopic methods and in comparison with those reported in the literature. This is the first report of these compounds from the plant. All four compounds from ethyl acetate showed quite high antioxidant activity, for which methyl gallate was the highest one. | Journal of Science and Technology 54 (4) (2016) 452-459 DOI: STUDY ON ANTIOXIDANT ACTIVITIES OF THE AERIAL PARTS AND SOME COMPOUNDS ISOLATED FROM Archidendron clypearia ((Jack) I. Niels Part 2. ISOLATING, DETERMINING STRUCTURE AND ANTIOXIDANT CAPABILITY OF SOME COMPOUNDS FROM ETHYL ACETATE AND CHLOROFORM EXTRACT Le Trung Hieu1, *, Vo Thi Mai Huong1, Nguyen Thi Hoai2, Tran Thi Van Thi1 1 2 The College of Sciences, Hue University, 77 Nguyen Hue, Hue city The College of Medicine and Pharmacy, Hue University, 41 Nguyen Hue, Hue city * Email: Received: 8 November 2015; Accepted for publication: 30 March 2016 ABSTRACT The antioxidant activity in vitro of methanol extract of Archidendron clypearia was evaluated by tests on isolated liver cells of mouses with ED50 value of µg/mL compared to that of curcumin of µg/mL. Using combined chromatographic methods, four compounds from ethyl acetate extract and two compounds from chloroform extract of the Archidendron clypearia were isolated. Their structures were elucidated to be daucosterol, 1-octacosanol, docosenoic acid, methyl gallate, betulinic acid and lup-20(29)-en-3-one by 1D- and 2D-NMR spectroscopic methods and in comparison with those reported in the literature. This is the first report of these compounds from the plant. All four compounds from ethyl acetate showed quite high antioxidant activity, for which methyl gallate was the highest one. Keywords: Archidendron clypearia, antioxidant activity, daucosterol, methyl gallate, 1octacosanol, acid docosenoic, betulinic acid, lup-20(29)-en-3-one. 1. INTRODUCTION Archidendron clypearia (Jack) I. Niels belongs to subfamily Mimosaceae. This originated from tropical countries as Vietnam, China. It is used in the folk medicine in Vietnam - Pako ethnic group for the treatment of diabetes, laryngitis, high blood pressure . [1]. As part of our research on the chemical constituents and biological activity of .
