On the one hand, it can tackle the safety issues, on the other hand, it can reserve FMDV intrinsic form which will provoke high immunogenicity. The important initial step to make virus-like particles is expression of the capsid proteins of FMDV in appropriate system. So in this study we describe how to design, express and purify of all capsid proteins: VP0, VP1 and VP3 of O type FMDV isolated in Vietnam field using SUMO fusion expression system. | Journal of Science and Technology 54 (5) (2016) 597-606 DOI: EXPRESSION AND PURIFICATION CAPSID PROTEINS VP0, VP1 AND VP3 OF FOOT AND MOUTH DISEASE VIRUS TYPE O IN ESCHERICHIA COLI Nguyen Hoang Duong1, *, Chi-Ning Chuang3, Nguyen Phuong Hoa1, Tran Thi Kim Dzung1, Le Thi Hong Minh2, Nguyen Mai Anh2, Nguyen Thi Kim Cuc2, Le Thu Ha 1, Pham Viet Cuong1, 2, Ting-Wang Fang3 1 Mientrung Institute for Scientific Research, Vietnam Academy of Science and Technology 2 Institute of Marine Biochemistry, Vietnam Academy of Science and Technology 3 Institute of Molecular Biology, Sinica Academia, Taiwan * Email: Received: 8 December 2015; Accepted for publication: 2 June 2016 ABSTRACT Foot and mouth disease virus (FMDV) causing infectious disease affects broadly clovenhoofed animals. It has 7 different serotypes. However, type O is the most prevalent in 3 founded types (O, A, Asia1) recurrence in Vietnam fields. The current vaccines used for FMDV is inactivated or attenuated forms. FMDV Vaccines were able to raise strong immune responses in host organisms, but still caused the safety concerns. Virus-like particles could be a new vaccine generation that fulfills the present questions. On the one hand, it can tackle the safety issues, on the other hand, it can reserve FMDV intrinsic form which will provoke high immunogenicity. The important initial step to make virus-like particles is expression of the capsid proteins of FMDV in appropriate system. So in this study we describe how to design, express and purify of all capsid proteins: VP0, VP1 and VP3 of O type FMDV isolated in Vietnam field using SUMO fusion expression system. Keywords: FMDV, SUMO, type O, vaccine, VLPs. 1. INTRODUCTION Foot and mouth disease (FMD) is endemic in many developing countries which mainly affects pigs, sheep, cattle and cloven-hoofed animals. FMD outbreaks cause huge economic losses through direct or indirect effects on agriculture, meat supply .
