Compound 11β,22-dihydroxyhopane was isolated from the methanol extract of the mycelium of Isaria japonica in Vietnam. Its structure was elucidated by spectral data analysis, including MS and 2D NMR. This is the first complete NMR assignment for 11β,22- dihydroxyhopane. | Vietnam Journal of Science and Technology 55 (5) (2017) 535-539 DOI: A HOPANE TRITERPENOID FROM THE MYCELIUM OF Isaria japonica IN VIETNAM Nguyen Ngoc Tuan1, Tran Thi Kim Anh1, Ping-Chung Kuo2, Tian-Shung Wu2, Tran Dinh Thang1, * 1 3 Department of Chemistry, Vinh University, Vinh City 42000, Vietnam School of Pharmacy, National Cheng Kung University, Tainan 70101, Taiwan, ROC * Email: thangtd@ Received: 3 January 2017; Accepted for publication: 2 June 2017 ABSTRACT Compound 11β,22-dihydroxyhopane was isolated from the methanol extract of the mycelium of Isaria japonica in Vietnam. Its structure was elucidated by spectral data analysis, including MS and 2D NMR. This is the first complete NMR assignment for 11β,22dihydroxyhopane. Keywords: Isaria japonica, triterpenoid, 11β, 22-dihydroxyhopane, NMR technique, mass spectrometry. 1. INTRODUCTION Cordyceps belongs to the family Clavicipitaceae of the order Hypocreales in the class Pyrenomycetes of ascomycetous fungi, and is known to be parasitized on insects [1, 2]. Various bioactive components were found in the genus Cordyceps. Isaria japonica is entomogenous fungi thought to be of the anamorph stage of Cordyceps [3]. This fungus with its host insects have been used in traditional medicine for various purposes in Japan, Korea and China [4, 5, 6]. Isaria japonica (Paecilomyces tenuipes) possesses anti-cancer activity in vivo [7] and significant cytotoxicity against cancer cell lines [8]. The culture liquid of Isaria japonica have been found to enhance the production of anti-sheep red blood cell IgM plaque-forming cells response upon oral administration in mice [6, 9]. In this article, we report the isolation and structural characterization of a hopane triterpenoid from the entomogenous fungi, Isaria japonica Yasuda. 2. EXPERIMENTAL General Melting point was measured on a Yanagimoto MP-S3 apparatus. NMR spectra were recorded on a Bruker AV-500 NMR spectrometer. ESI-MS .
