The ultimate goal of this paper is to develop derivatives of ferroceneservingas an electrochemical probe that would enhance the performance of immunosensor based on polypyrrole-streptavidin layer as platform for immobilization of biotinylatedferrocene and antibodies was developed. The measurement of redox signal of ferrocene was analyzed by differential pulse voltammetry (DPV) method and underlined a variation of redox properties upon the antigen interaction. The introduction of the redox biotinylatedferrocene into streptavidin-polypyrrole layer affords a good sensitivity of the biosensor with a detection limit of . | Journal of Science and Technology 54 (2C) (2016) 441-450 SYNTHESIS OF BIOTINYLATED FERROCENE, AND ITS APPLICATION AS A REDOX PROBE FOR ELECTROCHEMICAL IMMUNOSENSOR Le Huu Quynh Anh1, *, Phan Dinh Tuan1, HafsaKorri-Youssouffi2, Hélène Sauriat-Dorizon2 1 2 HochiminhCity University of Natural Resources and Environment, 236B Le Van Sy St., Tan Binh District, Hochiminh City, Vietnam ICMMO, CNRS, University of Paris-Sud15 Rue Georges Clemenceau, 91400 Orsay, France * Email: Received: 15 June 2016; Accepted for publication: 28 October 2016 ABSTRACT The coupling compound of Biotin (Vitamin H) and redox molecule ferrocene through covalent bonding was studied. Synthesis and structure identification of Biotinylated 1 - (- 2aminoethyl) ferrocene were performed by 1H-NMR, 13C-NMR and MS. The ultimate goal of this paper is to develop derivatives of ferroceneservingas an electrochemical probe that would enhance the performance of immunosensor based on polypyrrole-streptavidin layer as platform for immobilization of biotinylatedferrocene and antibodies was developed. The measurement of redox signal of ferrocene was analyzed by differential pulse voltammetry (DPV) method and underlined a variation of redox properties upon the antigen interaction. The introduction of the redox biotinylatedferrocene into streptavidin-polypyrrole layer affords a good sensitivity of the biosensor with a detection limit of . Keywords:immunosensor, ferrocene, redox probe, electrochemical detection, polypyrrole. 1. INTRODUCTION In recent years, electrochemical immunosensor has been studied and proven to be potential devices in many field such as clinical field [1], medicine and environment controlling, food safety [2]. These devices have many advantages such as high endurance, low cost, sensitivity and high selectivity, compact size, portability, simple manufacture process. The biosensors allow detecting biological molecules which are of small .
