This study used 3-day old zebrafish larvae (Danio rerio) as a model for toxicological testing. The semistatic testing was conducted to investigate the effects of different BPA concentrations (5 mg/L, 6 mg/L, 7 mg/L, 8 mg/L and 9 mg/L) that induced morphorlogical and physiological changes during the early development. | Journal of Science and Technology 54 (2A) (2016) 71-77 ACUTE TOXICITY OF BISPHENOL A INDUCED PHENOTYPIC CHANGES ON ZEBRAFISH (DANIO RERIO) DURING EARLY DEVELOPMENT Mai Ngo Thi1, *, Ngoc Vo Thi Kim2, Chi Do Hong Lan3, Nga Le Phi4 1 2 Department of Occupational Hygiene and Health, Sub- Institute of Labour and Environment Protection in the South of Vietnam Ho Chi Minh University of Science, Vietnam National University, 227 Nguyen Van Cu, District 5, Ho Chi Minh City, Vietnam 3 Institute of Environment and Resources, Vietnam National University, Ho Chi Minh City, Vietnam 4 Ho Chi Minh University of Technology, Vietnam National University Ho Chi Minh City, 268 Ly Thuong Kiet street, District 10, Ho Chi Minh City, Vietnam * Email: Received: 1 April 2016; Accepted for publication: 15 June 2016 ABSTRACT Bisphenol A (BPA) has been considered as a weak environmental estrogen, as similar to estradiol that has a potential in stimulating some cellular responses and phenotype changes. In addition, the ecological impacts of BPA to aquatic organisms have been increasingly raised at environmental relevant concentrations that potentially may affect to human health at early– life. This study used 3-day old zebrafish larvae (Danio rerio) as a model for toxicological testing. The semistatic testing was conducted to investigate the effects of different BPA concentrations (5 mg/L, 6 mg/L, 7 mg/L, 8 mg/L and 9 mg/L) that induced morphorlogical and physiological changes during the early development. As the results, the LC 50-24hrs, LC50-48hrs, LC50-72hrs and LC50-96hrs were determined as mg/L, mg/L, mg/L and mg/L, respectively. Phenotypic analysis revealed that toxicity caused cardiac edema. The result obtained from this research provided relevant information for environmental and human risk assessments. Keywords: Daniorerio, zebrafish, bisphenol A, acute toxicity. 1. INTRODUCTION Bisphenol A (BPA) is one of the highest produced .
