The fractionation of sugarcane bagasse using formic acid allowed removing lignin and hemicellulose, obtaining a material containing up to 90 % cellulose. The material can be easily hydrolyzed into glucose to serve as materials to produce high value added products such as biofuel, chemicals, pharmaceuticals, food additives, and the likes. | Journal of Science and Technology 54 (2A) (2016) 222-230 COMPARISON OF ETHANOL YIELD BETWEEN SEPARATE AND SIMULTANEOUS HYDROLYSIS AND ETHANOL FERMENTATION OF FORMIC- FRACTIONATED SUGARCANE BAGASSE Ngo Duy Sa1, To Kim Anh2, Pham Tuan Anh2, * 1 2 Department of Food Technology, Hatay Community College, Thuy Xuan Tien, Chuong My, Hanoi, Vietnam School of Biotechnology - Food Technology, Hanoi University of Science and Technology, No 1 Dai Co Viet Road, Hanoi, Vietnam * Email: Received: 1 April 2016; Accepted for publication: 15 June 2016 ABSTRACT The fractionation of sugarcane bagasse using formic acid allowed removing lignin and hemicellulose, obtaining a material containing up to 90 % cellulose. The material can be easily hydrolyzed into glucose to serve as materials to produce high value added products such as biofuel, chemicals, pharmaceuticals, food additives, and the likes. The hydrolysate of fractionated bagasse was easily fermented with a (ethanol) fermentation yield attained ± %, showing no significant inhibition to the yeast in the hydrolysate. In this study, a process of simultaneous hydrolysis and fermentation (SSF) was performed to convert fractionated sugarcane bagasse at 20 % consistency to ethanol. The process with 6h pre-hydrolysis at 50 0C then SSF at 37 0C could attain a high ethanol concentration of ± g/L in the fermentation with the ethanol recovery yield of ±; which ± % higher than that of the separate hydrolysis and fermentation (SHF) process ( ± %). In addition, in the SSF, the process time was shorten to 4 days instead of 7 days in the SHF. Keywords: bioethanol, cellulose, fractionation, hydrolysis and fermentation, sugarcane bagasse. 1. INTRODUCTION It was reported that production of sugar released ton sugarcane bagasse per every one ton of sugar [1] that contain high portion of cellulose (40 – 45 %) [2, 3]. There are more than 6-7 million tons of .
