The aim of this study was to evaluate the efficacy of whey protein isolate (WPI) as an encapsulation matrix for improvement of L. fermentum 39-183 viability to low pH and bile and releasing the encapsulated bacteria in ex vivo porcine gastrointestinal (GI) contents. | Kỷ yếu kỷ niệm 35 năm thành lập Trường ĐH ng nghiệp Th ph m T h inh -2017) USE OF WHEY PROTEIN FOR ENCAPSULATION AND CONTROLED RELEASE OF PROBITOIC FROM PROTEIN MICROENCAPSULATE IN EX VIVO PORCINE GASTROINTESTINAL CONTENTS Nguyen Thi My Le *, Nguyen Van Hieu, Pham Viet Nam Ho Chi Minh City University of Food Industry * Email: mylethang81@ Received: 20/08/2017; Accepted: 30/08/2017 ABSTRACT The aim of this study was to evaluate the efficacy of whey protein isolate (WPI) as an encapsulation matrix for improvement of L. fermentum 39-183 viability to low pH and bile and releasing the encapsulated bacteria in ex vivo porcine gastrointestinal (GI) contents. 1g of protein microcapsules (≈ 108 CFU of L. fermentum 39-183 or E. coli GFP+) were incubated in ex vivo porcine GI contents (9 mL) under anaerobic conditions at 37 ºC. Results showed that there was higher than 86% cell survival for encapsulated L. fermentum 39-183 after 3 h incubation in pH , whereas free cell experienced complete viability loss. Encapsulated L. fermentum 39-183 showed only about log reduction for all bile salt levels tested ( ÷ ), while log decrease of free cell after 6 h of incubation. There was almost a complete release ( x 108 CFU) of microencapsulated bacteria in the ileal contents within 2 h, while there was no significant release of encapsulated bacteria in the gastric contents even after 8 h of incubation. This study led to the development and design of a protein capsulation for reinforced probiotic protection during the stressful conditions of gastric and controlled release at a defined location. Keywords: Protein capsules, ex-vivo porcine gastrointestinal contents, lactobacillus fermentum 39-183, probiotics. 1. INTRODUCTION Microencapsulation with respect to a food application involves reversible of active bio-molecules in stable core and releasing it at desired sit (such as intestine or colon) in viable form. Probiotics, minerals, vitamins, .
