(BQ) Part 2 book “Radiobiology for the radiologist” has contents: Molecular techniques in radiobiology, cancer biology, clinical response of normal tissues, model tumor systems, alternative radiation modalities, the biology and exploitation of tumor hypoxia, and other contents. | For Students of Radiation Oncology 475 chapter 17 Molecular Techniques Radiobiology Historical Perspectives The Structure of DNA RNA and DNA Transcription and Translation The Genetic Code Amino Acids and Proteins Restriction Endonucleases Vectors Plasmids Bacteriophage λ Bacterial Artificial Chromosomes Viruses Libraries Genomic Library cDNA Library Hosts Escherichia Coli Yeast Mammalian Cells DNA-mediated Gene Transfer Agarose Gel Electrophoresis Polymerase Chain Reaction Polymerase Chain Reaction–mediated Site-directed Mutagenesis Gene-Cloning Strategies Genomic Analyses Mapping 476 in DNA Sequence Analyses Polymorphisms or Mutations Comparative Genome Hybridization Gene Knockout Strategies Clustered Regularly Interspaced Short Palindromic Repeats and CRISPR Associated Protein Homologous Recombination to Knockout Genes Knockout Mice Gene Expression Analysis Northern Blotting RNA Interference Reverse Transcription Polymerase Chain Reaction Quantitative Real-Time Polymerase Chain Reaction Genetic Reporters Promoter Bashing Chromatin Immunoprecipitation Protein–DNA Interaction Arrays (Chromatin Immunoprecipitation-Chips) Microarrays to Assay Gene Expression RNA-Seq to Assay Gene Expression Chromatin Immunoprecipitation-Seq Protein Analysis Western Blotting Antibody Production Immunoprecipitation Far Western Blotting Fluorescent Proteins Two-Hybrid Screening Split Luciferase Complementation Assay Proteomics Two-Dimensional Electrophoresis Databases and Sequence Analysis Summary of Pertinent Conclusions Glossary of Terms Bibliography 477 HISTORICAL PERSPECTIVES Recombinant DNA technology has revolutionized research in biology. It allows questions to be asked that were unthinkable just a few years ago. It is also a technology that is moving so fast that anything written in a book is likely to be out of date before it appears in print. This technology is invading every field of biologic research, and radiobiology is no exception. To keep abreast .
