Results also showed that the addition of proline ( g/l) or spermidine ( mM) to the culture medium increased the number of secondary embryos considerably. The fully developed plantlets exhibiting healthy roots and shoots were obtained when somatic embryos were sub-cultured onto B5 medium containing mg/l IBA. | Journal of Biotechnology 14(4): 661-671, 2016 PRIMARY AND SECONDARY SOMATIC EMBRYOGENESIS IN JATROPHA CURCAS L. FROM LEAF TRANSVERSE THIN CELL LAYERS Nguyen Thi Kim Loan1, Do Dang Giap1, Tran Trong Tuan1, Nguyen Thi Thanh Hien2, Nguyen Phuc Huy2, Thai Xuan Du1, Duong Tan Nhut2, * 1 2 Institute of Tropical Biology, Vietnam Academy of Science and Technology Tay Nguyen Institute for Scientific Research, Vietnam Academy of Science and Technology * To whom correspondence should be addressed. E-mail: duongtannhut@ Received: Accepted: SUMMARY An efficient method for plant regeneration in Jatropha curcas L. via primary and secondary somatic embryogenesis culture from ex vitro leaves of 6-month-old plants was presented in this study. Leaves were cut into transverse thin cell layers (tTCLs) and cultured on MS medium supplemented with kinetin (KIN) at , , , and mg/l in combination with indole-3-butyric acid (IBA) at , , and mg/l or 2,4dichlorophenoxyacetic acid (2,4-D) at , and mg/l . The highest embryogenic callus formation rate () was obtained on medium supplemented with mg/l KIN and mg/l 2,4-D. The calli were selected for the study of primary somatic embryogenesis on MS medium containing 2,4-D (, , , and mg/l) or KIN (, , , and mg/l). The highest primary somatic embryos formation rate () was achieved on MS medium supplemented with mg/l KIN. The primary embryos were cultured on medium supplemented with KIN (, , , , and mg/l) combined with mg/l indole-3-butyric acid (IBA) or mg/l 2,4-D. The combination of mg/l KIN and mg/l 2,4-D was suitable for secondary embryos formation. Embryos proliferated rapidly, and the highest number of secondary embryos ( embryos) wasobtained from a single primary embryos inoculated. Results also showed that the addition of proline ( g/l) or spermidine ( mM) to the culture medium .
