Using various chromatographic separations, three cholestane-type steroids were isolated from the methanol extract of the octocoral Verrucella corona. Their structures were elucidated to be (22E)-cholesta-5,22-dien-3β-ol-7-one (1), trans-liagosterol (2), and guggulsterol-II (3), by detailed analysis of the 1D and 2D-NMR data as well as comparison with those reported. Among them, compound 2 showed significant cytotoxicity against eight human cancer cell lines as HepG2, HL-60, KB, LNCaP, LU-1, MCF7, SK-Mel2, and SW480. | Vietnam Journal of Science and Technology 56 (4A) (2018) 279-285 CHOLESTANE-TYPE STEROIDS FROM THE OCTOCORAL VERRUCELLA CORONA Ninh Thi Ngoc1, Tran Thi Hong Hanh1, Nguyen Xuan Cuong1, *, Nguyen Van Thanh1, Do Thi Thao2, Nguyen Hoai Nam1, Do Cong Thung3, Phan Van Kiem1, Chau Van Minh1 1 Institute of Marine Biochemistry (IMBC), Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Ha Noi 2 Institute of Biotechnology (IBT), VAST, 18 Hoang Quoc Viet, Ha Noi 3 Institute of Marine Environment and Resources (IMER), VAST, 246 Da Nang, Hai Phong * Email: cuongnx@ Received: 23 July 2018, Accepted for publication: 14 October 2018 ABSTRACT Using various chromatographic separations, three cholestane-type steroids were isolated from the methanol extract of the octocoral Verrucella corona. Their structures were elucidated to be (22E)-cholesta-5,22-dien-3β-ol-7-one (1), trans-liagosterol (2), and guggulsterol-II (3), by detailed analysis of the 1D and 2D-NMR data as well as comparison with those reported. Among them, compound 2 showed significant cytotoxicity against eight human cancer cell lines as HepG2, HL-60, KB, LNCaP, LU-1, MCF7, SK-Mel2, and SW480. Keywords: Verrucella corona, octocoral, steroid, cytotoxic activity. 1. INTRODUCTION Verrucella corona is an octocoral belonging to Ellisellidae family, Alcyonacea order, Octocorallia subclass, Anthozoa class, and Cnidaria phylum. Verrucella is a little investigated genus with some papers reported the isolation of steroid [1, 2], briarane-type diterpenoid [3], and N-atom-containing constituents [4]. Recently, we have reported seven new steroids from V. corona and their in vitro cytotoxic effects [5]. In this paper, we further report the isolation, structural elucidation, and cytotoxic activity of three steroids as (22E)-cholesta-5,22-dien-3β-ol7-one (1), trans-liagosterol (2), and guggulsterol-II (3) from this species. 2. EXPERIMENTAL . General methods Optical rotations were measured on a .
