In the present work, ultrasonic-assisted enzymatic extraction (UAEE) was used for extraction of PS from Lingzhi. The experiments were conducted according to a Box-Behnken design (BBD), with four independent variables: extraction temperature, ultrasonic power, pH, and temperature time. | Vietnam Journal of Science and Technology 56 (4A) (2018) 171-181 EXTRACTION OF POLYSACCHARIDES FROM LINGZHI BY ULTRASONIC-ASSISTED ENZYMATIC METHOD Tran Quoc Hoa1, Tran Thi Phuong Mai2, Hoang Minh Nam1, 2, Nguyen Huu Hieu1, 2, * 1 Key Laboratory of Chemical Engineering and Petroleum Processing, Ho Chi Minh City University of Technology, VNU-HCM, 268 Ly ThuongKiet Street, Ward 14, District 10,Ho Chi Minh City 2 Faculty of Chemical Engineering, Ho Chi Minh City University of Technology, VNU-HCM * Email: nhhieubk@ Received: 23 July 2018; Accepted for publication: 9 October 2018 ABSTRACT The medicinal values of polysaccharides (PS) in Lingzhi have been shown to lie in many anti-cancer effects and good benefits for human health. Lingzhi, which is rich in healthy PS, has been used more and more commonly in Viet Nam in recent years. In the present work, ultrasonic-assisted enzymatic extraction (UAEE) was used for extraction of PS from Lingzhi. The experiments were conducted according to a Box-Behnken design (BBD), with four independent variables: extraction temperature, ultrasonic power, pH, and temperature time. The results showed that the best adequate extraction conditions were extraction time of 144 min, extraction temperature of 55 °C, ultrasonic power of 240 W, pH , and temperature time of 144 min. Under these conditions, the predicted optimal yield was %. Whereas by following the optimized condition, the experimental yield of PS was ± %, which was in good agreement with that of the prediction. Compared to the hot water extraction (HWE) method, ultrasonic-assisted extraction (UAE) method and enzyme-assisted extraction (EAE) method, the yield of PS obtained by UAEE was favorable. The PS yield obtained by HWE, and EAE were % and %, respectively. These results demonstrated that UAEE was an appropriate and effective extraction of polysaccharides from Lingzhi. Keywords:Lingzhi,ultrasonic-assisted enzymatic extraction, .
