This study assessed and compared the in vitro cytotoxic activity of crude extracts from fruit bodies and biomass of C. neovolkiana fungus against human Jurkat (acute T cell leukemia) and breast carcinoma (MCF-7) cells using the sulforhodamine B (SRB) assay. | Vietnam Journal of Science and Technology 56 (4A) (2018) 53-60 A COMPARISON OF THE CYTOTOXIC ACTIVITY OF EXTRACTS FROM FRUITING BODIES AND MYCELIAL BIOMASS OF CORDYCEPS NEOVOLKIANA (DL004) FUNGUS Nguyen Chi Dung1, 2, *, Pham Thi My Ninh3, Dinh Minh Hiep2 1 Institute of Tropical Biology, Vietnam Academy of Science and Technology, 9/621 Ha Noi highway, Linh Trung Ward, Thu Duc District, Ho Chi Minh City 2 Management Board of Agricultural Hi-Tech Park,214, D5 Street, Ward 25, Binh Thanh District, Ho Chi Minh City 3 University of Science, Vietnam National University, 227, Nguyen Van Cu street, Ward 4, District 5, Ho Chi Minh City * Email: dungnguyen1507@ Received: 7 August 2018; Accepted for publication: 13 October 2018 ABSTRACT Cordyceps neovolkiana is an insect-parasitic fungus, naturally distributed in the Langbiang mountain, Tam Dao and Ba Vi national parks of Vietnam. This study assessed and compared the in vitro cytotoxic activity of crude extracts from fruit bodies and biomass of C. neovolkiana fungus against human Jurkat (acute T cell leukemia) and breast carcinoma (MCF-7) cells using the sulforhodamine B (SRB) assay. We obtained the extracts via the ethanol extraction and liquid-liquid extraction with four different solvents:ethanol (EtOH) petroleum ether (60 – 80 oC) (PE), ethyl acetate (EA), butanol (BU) and water (W), successively. The result shows that the cytotoxic potential of biomass and fruit-body extracts were determined by the IC50 value at 0 – 100 µg/mL concentration. The PE-fruit-body extract displayed the highest cytotoxic activity against MCF-7 and Jurkat cell lines with IC50 values of ± µg/ml and ± µg/ml, respectively. Besides, the PE- biomass extracts showed the highest cytotoxic activity against CF-7 and Jurkat cell lines with IC50 values of ± µg/mL and ± µg/ml, respectively. Obviously, we need to study further about the cytotoxic mechanism PE biomass extracts on MCF-7 and Jurkat .
