The results illustrated that, in the RBO-added media, the biomass of mycelial and EPS production increased significantly compared to non-RBO medium. In particular, biomass yield was g/L in medium within 6% RBO, and EPS production was g/L in medium within 5 % RBO. | Vietnam Journal of Science and Technology 56 (4A) (2018) 74-82 EFFECT OF RICE BRAN OIL ON MYCELIAL BIOMASS PRODUCTION, BIOSYNTHESIS AND BIOACTIVITIES OF POLYSACCHARIDES BY OPHIOCORDYCEPS SINENSIS FUNGUS Ha Bao Chau1, Nguyen Tai Hoang1, *, Nguyen Thi Tra My1, Bui Lap Duy1, Tran Van Hai Nam1, Ha Thi Bich Hang1, Le Thi Thuy Hang2, 3, Dinh Minh Hiep4 1 Ho Chi Minh City University of Science, 227, Nguyen Van Cu St., Ho Chi Minh City 2 Ho Chi Minh City University of Food Industry, 140 Le Trong Tan, Ho Chi Minh City 3 Vietnam Academy of Science and Technology, 18, Hoang Quoc Viet, Ha Noi 4 Agricultural Hi-Tech Park of Ho Chi Minh City, 214-214A, D5St., Ho Chi Minh City * Email: hoangnguyenhoang191@ Received: 8 August 2018; Accepted for publication: 10 October 2018 ABSTRACT Ophiocordyceps sinensis is an entomopathogenic fungus, which is one of the most valued natural remedies in Traditional Chinese Medicine. It continues to attract scientists’ attention due tothe diversity of bioactive compounds such as polysaccharides, cordycepin, adenosine, andergosterol. Among them, exopolysaccharides (EPS) from the culture broth has possessedmany precious bioactivities, for example, immunomodulatory, antitumor, antioxidant activity. Interestingly, previous studies have demonstrated that plant oils have positive affects to the growth and biosynthesis of second darymetabolites of medicinal fungi. Therefore, the aim of this study is to enhance the biomass growth and EPS biosynthesis of O. sinensis fungus by implementing rice bran oil (RBO) at various concentrations from 1 % to 8 % (v/v) into the culture medium. The results illustrated that, in the RBO-added media, the biomass of mycelial and EPS production increased significantly compared to non-RBO medium. In particular, biomass yield was g/L in medium within 6% RBO, and EPS production was g/L in medium within 5 % RBO. Importantly, inhibition of xanthine oxidase activity of the IPS (2 % RBO) and EPS (8 % RBO)
