Callicarpa macrophylla Vahl. (Tu Chau la to) is usually used in Vietnam traditional medicine for treating gastrointestinal bleeding, nosebleeds; in addition, it is also used for treating osteoarthritis pain. Phytochemical investigation of hexane and ethyl acetate extracts of leaves Callicarpa macrophylla Vahl led to the isolation of β-sitosterol (1), β-sitosterol-3-O-β-Dglucopyranoside (2), stigmasta-7,22-dien-3β-ol (3), β-amyrin (4), ent-1α-acetoxy-7β,14αdihydroxy-kaur-16-en-15-on (5) and ent-7β,16,17,18-tetrahydroxy-kaurane-15-on (6). | Vietnam Journal of Science and Technology 56 (4A) (2018) 213-220 TERPENE COMPOUNDS ISOLATED FROM CALLICARPA MACROPHYLLA IN VIET NAM Do Tien Lam1, 2, Pham Minh Quan1, 2, Vu Thi Thu Le1, 2, Nguyen Thi Hong Van1, Cam Thi Inh1, Ta Thi Thu Thuy3, Pham Thi Hong Minh1, 2, * 1 Institute of Natural Products Chemistry, Vietnam Academy of Science and Technology, No. 18 Hoang Quoc Viet, Cau Giay, Ha Noi 2 Graduate University of Science and Technology, Vietnam Academy of Science and Technology, No. 18 Hoang Quoc Viet, Cau Giay, Ha Noi 3 Faculty of Biotechnology, Hanoi Open University, B101 Nguyen HienHa Noi * Email: minhhcsh@ Received: 2 September 2018; Accepted for publication: 6 October 2018 ABSTRACT Callicarpa macrophylla Vahl. (Tu Chau la to) is usually used in Vietnam traditional medicine for treating gastrointestinal bleeding, nosebleeds; in addition, it is also used for treating osteoarthritis pain. Phytochemical investigation of hexane and ethyl acetate extracts of leaves Callicarpa macrophylla Vahl led to the isolation of β-sitosterol (1), β-sitosterol-3-O-β-Dglucopyranoside (2), stigmasta-7,22-dien-3β-ol (3), β-amyrin (4), ent-1α-acetoxy-7β,14αdihydroxy-kaur-16-en-15-on (5) and ent-7β,16,17,18-tetrahydroxy-kaurane-15-on (6). Their chemical structures were determined by spectroscopic methods including MS, 1D, 2D NMR and comparing with those reported in previous papers. Three compounds 4, 5, 6 were isolated for the first time from Callicarpa macrophylla plant. Keywords: Callicarpa macrophylla Vahl., sterol, triterpene and diterpene. 1. INTRODUCTION Tu chau la to or called as Tu hu la to or Bot ech la to has the scientific name as Callicarpa macrophylla Vahl. or Callicarpa cana L. belong to Verbenaceae family, it is distributed mainly in Vietnam, Thailand, Myanmar, India, China, Nepal, etc. [1, 2]. In Viet Nam, the plant grows wild in mountainous regions such as the northern provinces of Son La, Quang Ninh, Ha Tay, Ninh Binh, Nghe An [1, 2]. According to
