The phenotypic changes in target cell induced by an exogenous agent, . biologically active chemical, can be detected by labelling selected organelles with fluorescent dyes and observed under the high-throughput microscope. The changes of known biological targets (. nucleus, proteins, mitochondria, microtubules, etc.) | Vietnam Journal of Science and Technology 56 (4A) (2018) 1-12 Mini Review APPLICATION OF IMAGE-BASED HIGH CONTENT ANALYSIS FOR THE SCREENING OF BIOACTIVE NATURAL-PRODUCTS Do Huu Nghi1, 2, *, Le Mai Huong1, 2, Le Huu Cuong1, 2, Do Huu Chi3, Vu Dinh Giap1, 2, Nguyen Manh Cuong1, 2 1 Institute of Natural Products Chemistry, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Ha Noi, Viet Nam 2 Graduate University of Science and Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Ha Noi, Viet Nam 3 Department of Cellular & Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu city, Shizuoka, Japan * Email: nghi@ Received: 20/9/2018; Accepted for publication: 14 October 2018 ABSTRACT The phenotypic changes in target cell induced by an exogenous agent, . biologically active chemical, can be detected by labelling selected organelles with fluorescent dyes and observed under the high-throughput microscope. The changes of known biological targets (. nucleus, proteins, mitochondria, microtubules, etc.) induced by a compound of interest allow predicting its mechanisms of action. High-throughput microscopy with capability of multiparametric imaging in combination with automated image-analysis is referred to as highcontent analysis or high-content screening (HCS). Recently, HCS plays an increasingly important role for a large-scale screening of small molecules for several aspects including anticancer, anti-inflammatory activities, disease-related biomarkers and in vitro toxicity testing, and so on. As an introduction, this short review covers the concepts of HCS and its application in screening of biologically active natural products whose molecular targets could be identified through such approaches. Keywords: cell-based assay, high-content screening, automated microscope, imaging technology, bioactive natural-products. 1. INTRODUCTION Natural products have largely been .
