Ruellia tuberosa L. is a folk remedy, which is used in the treatment of diabetes mellitus, antiinflammation and diuretic agent. However, its α-glucosidase inhibitory activity has not been investigated so far. In the present study, the antidiabetic activitiesof the hexane, ethyl acetate and methanol fraction of crude ethanolic extract of this plant were investigated. | Vietnam Journal of Science and Technology 56 (4A) (2018) 106-112 RESEARCH ON PHYTOCHEMICAL AND ALFA-GLUCOSIDASE INHIBITORY ACTIVITY OF ETHYL ACETATE FRACTION OF RUELLIA TUBEROSA Pham Thi Nhat Trinh1, Pham Ngoc An2, Nguyen Trong Tuan3, Nguyen Thi Le Thuy4, Tong Thanh Danh5, Le Tien Dung2, 6, * Faculty of Natural Sciences, Tien Giang University, 119 Ap Bac – My Tho – Tien Giang 2 Institute of Applied Materials Science, VAST,01A TL29, , Ho Chi Minh city 1 3 Department of Chemistry, College of Natural Sciences,Can Tho University, Dist. Ninh Kieu, Can Tho 4 Department of Food Technology, Faculty of Biotechnology, Open University Ho Chi Minh City, 97 Vo Van Tan, Dist. 3, Ho Chi Minh City 5 Department of Organic Chemistry, Faculty of Chemical Engineering, Ho Chi Minh City University of Technology, 268 Ly Thuong Kiet, Dist. 10, Ho Chi Minh City 6 Graduate University of Science and Technology, VAST, 18 Hoang Quoc Viet, Cau Giay, Ha Noi * Email: inpcdung@ Received: 20 September 2018; Accepted for publication: 10 October 2018 ABSTRACT Ruellia tuberosa L. is a folk remedy, which is used in the treatment of diabetes mellitus, antiinflammation and diuretic agent. However, its α-glucosidase inhibitory activity has not been investigated so far. In the present study, the antidiabetic activitiesof the hexane, ethyl acetate and methanol fraction of crude ethanolic extract of this plant were investigated. Our preliminary results showed that the crude ethanol extract as well as ethyl acetate and methanol fractions of Ruellia tuberosa showed α-glucosidase inhibitory activity with IC50 of , and µg/ml, respectively. From the ethyl acetate extract of whole plant of Ruellia tuberosa, four compounds, including rutin (1), syringaresinol (2), catechin (3) and pulmatin (4) were isolated and identified by column chromatography. Their structures were elucidated by spectroscopic method including MS, 1D and 2D- NMR and comparison with the literature .
