The media contain laccase with the activity of . Moreover, Pleurotus sp. also grew in the media which contain glyphosate and made a decrease in this content from 50mg/kg to after 12 days. | Tạp chí Phát triển Khoa học và Công nghệ, tập 20, số K3-2017 147 Solid-state production of laccase by Pleurotus sp. applied in glyphosate degradation Do Thi Truc Ly, Le Hoai Diem Phuong, Nguyen Minh Quang, Luong Bao Uyen Abstract— Nphosphonomethyl glycine (glyphosate) is the derivative of the amino acid glycine. Glyphosate is a broad-spectrum herbicide. Glyphosate is translocated via phloem system and moves from the roots to the growing parts of the plants. Many researches proved that ligninmodifying enzymes LME including laccases have ability to degrade glyphosate. Pleurotus sp. was cultured during 12 days in the media with coir dust, sesame residue, urea, saccharose, K 2HPO4 and minerals. After 12 days, the media contain laccase with the activity of . Moreover, Pleurotus sp. also grew in the media which contain glyphosate and made a decrease in this content from 50mg/kg to after 12 days. Index Terms— glyphosate degradation, Pleurotus, laccase. 1 INTRODUCTION W ith the advance in technology to clear grass or control pest and plant diseases, we are using the chemicals which are the one of many reasons for the argicultural pollution and soil contamination. Laccases with its advantages have being used in many researches to degrade xenbiotic which are difficult to naturally decompose. Laccases were studied in decompostion of herbicides or pesticides such as atrazine, DDT, phenyl urea, 2,4,6-trichlorophenyl p-nitrophenyl, p-benzoquinone, [1,3], halogenated pesticide , 2,4-D, dioxin [2], and glyphosate [7]. Manuscript Received on March 15th, 2017, Manuscript Revised on November 01st, 2017. The authors are grateful to the Department of Science and Technology of Ho Chi Minh City for financial support. Do Thi Truc Ly, University of Science, VNU-HCM. Le Hoai Diem Phuong, University of Science, VNU-HCM. Nguyen Minh Quang, Biochemistry Laboratory (e-mail: nmquang93@). Luong Bao Uyen, University of Science, VNU-HCM (email: .
