Application of chemical mutagens as important factors is very practical for successful haploidization techniques in Cucurbitaceae through pollen grain deactivation and then immature embryo rescue. | Turkish Journal of Agriculture and Forestry Research Article Turk J Agric For (2018) 42: 11-21 © TÜBİTAK doi: Use of chemical mutagens for production of inactive pollen grains, embryo rescue, and morphological changes in cucumber Faezeh HAZEM, Maryam GOLABADI* Department of Agronomy and Plant Breeding, College of Agriculture, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran Received: Accepted/Published Online: Final Version: Abstract: Application of chemical mutagens as important factors is very practical for successful haploidization techniques in Cucurbitaceae through pollen grain deactivation and then immature embryo rescue. The present research investigated the effects of genotype, male flower age, receptor plant, and chemical mutagenesis (NaN3 and colchicine) on pollen grain deactivation as well as seed production (number of total, full, half-full, and empty seeds) and morphological traits. Moreover, the effects of different plant growth regulators were tested on embryo cultures. The effects of different factors (mutagen, genotype, flower age) were investigated on plant regeneration from immature embryos that were inspected from half-full and empty seeds. Based on this study, some mutagenic treatments ( colchicine and NaN3) led to the highest values of morphological and fruit yield traits in control plants, while and NaN3 led to reduced values of these traits. Among the genotypes, NBDC3 showed the highest number of different types of seeds. Among different chemical mutagens, NaN3 ( mg/L) and colchicine ( mg/L) produced the greatest effects on seed production traits. Moreover, the highest values for all seed-related traits were recorded for crossing with fresh male flowers, whereas crossing with old male flowers contributed to the decline of seed-related traits. Mutagen-treated plants showed the highest empty
