The present study investigates fatty acid composition and trans fatty acid formation in oils extracted from French-fried potatoes, which were produced in the laboratory and collected from different restaurants. | Turkish Journal of Agriculture and Forestry Turk J Agric For (2015) 39: 80-90 © TÜBİTAK doi: Research Article Investigation of fatty acid composition and trans fatty acid formation in extracted oils from French-fried potatoes and classification of samples using chemometric approaches 1 2 1, 3 1 Elif YILDIRIM , Ömer Said TOKER , Safa KARAMAN *, Ahmed KAYACIER , Mahmut DOĞAN 1 Food Engineering Department, Engineering Faculty, Erciyes University, Kayseri, Turkey 2 Food Engineering Department, Chemical and Metallurgical Engineering Faculty, Yıldız Technical University, İstanbul, Turkey 3 Food Engineering Department, Natural Sciences, Architecture, and Engineering Faculty, Bursa Technical University, Bursa, Turkey Received: Accepted: Published Online: Printed: Abstract: The present study investigates fatty acid composition and trans fatty acid formation in oils extracted from French-fried potatoes, which were produced in the laboratory and collected from different restaurants. Potatoes were fried at 180 °C at different frying periods (1, 7, 13, 19, 25, and 31 min) and the fatty acid composition of the extracted oils was determined. Principal component analysis and hierarchical cluster analysis were used to characterize and classify the samples. Four major fatty acids were identified in the samples, namely palmitic acid, stearic acid, oleic acid, and linoleic acid. Linolelaidic acid (C18:2 trans) was detected in the oil samples extracted from potatoes fried in margarine. Three principal components (PCs) and 4 main clusters were obtained from the chemometric analysis, which characterized the samples. Three PCs were found to be explanatory of more than of the total variability in the data set. Key words: Fatty acid composition, French-fried potatoes, trans configuration, principal component analysis, hierarchical cluster analysis 1. .