Since the plgms19 and STS-834b primers were found to be effective at high ratios in determining resistance to RKN in the Nemaguard × GF677 F1 population, the use of these markers could be included in breeding studies. It was also detected that the STS-OPS14a primer is appropriate to use for determining the sensitivity to RKN in almond rootstocks. | Turkish Journal of Agriculture and Forestry Turk J Agric For (2015) 39: 563-571 © TÜBİTAK doi: Research Article Investigation of root-knot nematode (Meloidogyne spp.) resistance in almond rootstocks with DNA markers 1 2 3 4 1, Sultan BAY TÜRKOĞLU , Nergiz ÇOBAN , Ali ÖZKAN , Halit Seyfettin ATLI , Canan CAN * 1 Department of Biology, Faculty of Arts and Sciences, Gaziantep University, Gaziantep, Turkey 2 Pistachio Research Station, Ministry of Food, Agriculture, and Livestock, Gaziantep, Turkey 3 Department of Gastronomy and Culinary Arts, Faculty of Fine Arts, Gaziantep University, Gaziantep, Turkey 4 Department of Horticulture, Faculty of Agriculture, Siirt University, Siirt, Turkey Received: Accepted: Published Online: Printed: Abstract: Seven almond varieties, Ferragnes, Nemaguard, AB3 (Amygdalus orientalis Mill.), GN22, GF677, Myrobalan (Prunus cerasifera), and Pissardi nigra, and F1 crossbreed almonds, Ferragnes × Pissardi nigra, Ferragnes × Myrobalan, Ferragnes × GN22, Nemaguard × GF677, Myrobalan × AB3 (Prunus dulcis Mill.), Myrobalan × Ferragnes, Pissardi nigra × AB3, and GF677 × Myrobalan, were used to determine resistance against root-knot nematode (RKN) (Meloidogyne spp.) using DNA markers. Parental lines and 316 F1 plants were tested with SSR (plgms8, plgms9, and plgms19) and STS (STS-OPS14a, STS-834b) markers. It was found that plgms19 exhibited 76%, , , and inheritance of resistance in the Ferragnes × Pissardi nigra, Ferragnes × Myrobalan, Ferragnes × GN22, and Nemaguard × GF677 F1 populations, respectively. No resistance was detected in the Nemaguard × GF677 population with the plgms8 marker. Since the plgms19 and STS-834b primers were found to be effective at high ratios in determining resistance to RKN in the Nemaguard × GF677 F1 population, the use of these markers could be included in breeding studies. It was
