V-myb avian myeloblastosis viral oncogene homolog (MYB) transcription factors play important roles in the processes of plant growth, development, and stress responses. In this study, a full-length cDNA sequence of a MYB gene, designated FvMYB1, was isolated from Arizona ash (Fraxinus velutina Torr.) for the first time. | Turkish Journal of Agriculture and Forestry Turk J Agric For (2013) 37: 517-526 © TÜBİTAK doi: Research Article Molecular cloning and expression analysis of FvMYB1 from Fraxinus velutina Torr. 1,2,3 2 1,2, 2 Tian LI , Zhen-Ying PENG , Yu-Ping BI *, Zhong-Xue FAN College of Life Science, Shandong Normal University, Shandong, . China 2 Shandong Provincial Key Laboratory of Genetic Improvement, Ecology and Physiology of Crops and Key Laboratory of Crop Genetic Improvement and Biotechnology, Huanghuaihai, Ministry of Agriculture, Hi-Tech Research Center, Shandong Academy of Agricultural Science, Shandong, . China 3 Shandong Provincial Key Laboratory of Eco-Environmental Science for Yellow River Delta, Binzhou University, Shandong, . China 1 Received: Accepted: Published Online: Printed: Abstract: V-myb avian myeloblastosis viral oncogene homolog (MYB) transcription factors play important roles in the processes of plant growth, development, and stress responses. In this study, a full-length cDNA sequence of a MYB gene, designated FvMYB1, was isolated from Arizona ash (Fraxinus velutina Torr.) for the first time. Sequence analysis showed that the deduced amino acid sequence of FvMYB1 encoded novel MYB proteins with single DNA binding domains. Based on the R3 domain amino acid sequence, the FvMYB1 was closely related to some proteins whose functions were known. The expression pattern of FvMYB1 in different organs of Arizona ash was analyzed by semiquantitative RT-PCR and real-time PCR (qRT-PCR). Results showed that this gene was widely distributed in all the tested organs and the expression level of FvMYB1 was the highest in stems and the lowest in roots. The gene expression level decreased dramatically in roots under salt treatment (300 mM, 24 h), while no obvious change was observed in stems. Subcellular localization indicated that FvMYB1 was .
