In the present study, the effects of indole-3-butyric acid (IBA) alone and in combination with L-methionine were investigated on the morphogenic and biochemical responses in the shoot tip explants of the cherry rootstocks CAB-6P (Prunus cerasus L.) and Gisela 6 (Prunus cerasus × Prunus canescens). | Turkish Journal of Agriculture and Forestry Research Article Turk J Agric For (2013) 37: 688-698 © TÜBİTAK doi: In vitro rooting and biochemical parameters in the cherry rootstocks CAB-6P and Gisela 6 using L-methionine Virginia SARROPOULOU*, Kortessa DIMASSI-THERIOU, Ioannis THERIOS Laboratory of Pomology, Department of Horticulture, School of Agriculture, Aristotle University of Thessaloniki, Thessaloniki, Greece Received: Accepted: Published Online: Printed: Abstract: In the present study, the effects of indole-3-butyric acid (IBA) alone and in combination with L-methionine were investigated on the morphogenic and biochemical responses in the shoot tip explants of the cherry rootstocks CAB-6P (Prunus cerasus L.) and Gisela 6 (Prunus cerasus × Prunus canescens). The best results for roots, regarding their number and the fresh and dry weight, were obtained with 2 mg L–1 IBA in both rootstocks. Maximum rooting percentage was with the combination of 2 mg L–1 IBA and mg L–1 L-methionine. Root length was greatest in the control plants. L-methionine had an inhibitory effect on the leaf chlorophyll content of the CAB-6P rootstock and no effect in Gisela 6. L-methionine with the lowest IBA concentration ( mg L–1) exhibited elevated levels of proline in the roots of the CAB-6P rootstock and depleted levels in Gisela 6. For the Gisela 6 rootstock, the leaf carbohydrate concentration was highest with 2 mg L–1 of IBA, whereas in the roots it was highest in the control. For the CAB-6P rootstock, the leaf carbohydrate concentration was not influenced by IBA with L-methionine, whereas the highest carbohydrate concentration in the roots was with the combinations of mg L–1 IBA and 1 and 2 mg L–1 L-methionine. Taking into account the different parameters examined, it appears that chlorophyll and carbohydrates are the most accurate biochemical markers of the
